Sequencing and Phylogenetic Analysis of Infectious Bovine Rhinotracheitis Virus Gb and Gd Genes in Iran
نویسنده
چکیده
Bovine herpesvirus 1 (BHV-1) belongs to the Varicellovirus genus, Herpesviridae family and causes different respiratory, reproductive and nervous system disorders in cows, including infectious bovine rhinotracheitis (IBR). For determination of the genetic relationship of gB and gD – two of main BHV-1 glycoprotein genes in Iran with those in other countries, DNA fragments of 671 bp corresponding to gB and 1002 bp corresponding to gD from four IBR viral strains have been isolated from bovine respiratory disorders in Iran, then were amplified in PCR system, sequenced for determining nucleotide sequence and compared with identified nucleotide sequences of these genes in other countries. The results indicated 6.6% to 14.5% variability in gB gene and 1.1% to 13.3% variability in gD gene in four sequenced samples. A comparison made on gB and gD genes in Iran with other countries showed 6.4% to 59% variability in gB gene and 8.7% to 29.2% variability in gD gene.
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تاریخ انتشار 2009